Keratins 17 and 19 expression as prognostic markers in oral squamous cell carcinoma.

Five-year survival rates for oral squamous cell carcinoma (OSCC) are 30% and the mortality rate is 50%. Immunohistochemistry panels are used to evaluate proliferation, vascularization, apoptosis, HPV infection, and keratin expression, which are important markers of malignant progression. Keratins are a family of intermediate filaments predominantly expressed in epithelial cells and have an essential role in mechanical support and cytoskeleton formation, which is essential for the structural integrity and stability of the cell. In this study, we analyzed the expressions of keratins 17 and 19 (K17 and K19) by immunohistochemistry in tumoral and non-tumoral tissues from patients with OSCC. The results show that expression of these keratins is higher in tumor tissues compared to non-tumor tissues. Positive K17 expression correlates with lymph node metastasis and multivariate analysis confirmed this relationship, revealing a 6-fold increase in lymph node metastasis when K17 is expressed. We observed a correlation between K17 expression with disease-free survival and disease-specific death in patients who received surgery and radiotherapy. Multivariate analysis revealed that low expression of K17 was an independent marker for early disease relapse and disease-specific death in patients treated with surgery and radiotherapy, with an approximately 4-fold increased risk when compared to high K17 expression. Our results suggest a potential role for K17 and K19 expression profiles as tumor prognostic markers in OSCC patients.

Eficácia In Vitro De Extratos De Chenopodium Ambrosioides Sobre Teleóginas De Rhipicephalus (Boophilus) Microplus / In vitro efficacy of Chenopodium ambrosioides extracts on Rhipicephalus (Boophilus) microplus engorged females

Chenopodium ambrosioides L. (Amaranthaceae) (erva-de-santa-maria) é uma planta fortemente aromática usada popularmente por suas propriedades antiparasitárias.Avaliou-se com este estudo a eficácia in vitro de extratos hidroetanólicos de C. ambrosioides sobre a postura e a eclodibilidade larval de Rhipicephalus (Boophilus) microplus (Canestrini, 1888) (Acari: Ixodidae), usando o teste de imersão de fêmeas ingurgitadas adultas.Os tratamentos foram constituídos por extratos feitos com 5%, 10% e 25% de C. ambrosioides (massa/volume), água destilada e veículo (constituído de propilenoglicol a 5%, etanol a 25% e água destilada). Dez teleóginas foram distribuídas de forma homogênea para cada grupo e imersas por 5 minutos em cada um dos extratos testados. Os extratos feitos com 5%, 10% e 25% de C. ambrosioides apresentaram eficácias médias de 13,27%, 22,56% e 31,87%, respectivamente. Estes resultados indicam que, nas concentrações usadas, os extratos de C. ambrosioides não apresentam potencial para o controle das cepas pesquisadas de R. (B.) microplus.

In vitro efficacy of Chenopodium ambrosioides extracts on Rhipicephalus (Boophilus) microplus engorged females. Chenopodium ambrosioides L. (Amaranthaceae) (wormseed) is a strongly aromatic plant employed popularly for its antiparasitic properties. This study evaluated the in vitro efficacy on laying and hatchability of eggs of Rhipicephalus (Boophilus) microplus (Canestrini, 1888) (Acari: Ixodidae) of hydroethanolic extracts from C. ambrosioides, using the adult immersion test. The treatments consisted of hydroethanolic extracts made with 5%, 10% and 25% of C. ambrosioides (weight/volume), distilled water and vehicle solution (consisting of propylene glycol at 5%, ethanol at 25% and distilled water). Ten ticks were distributed evenly for each group and immersed for 5 minutes in each of the tested extracts. The extracts containing 5%, 10% and 25% of C. ambrosioides showed average efficacies 13.27%, 22.56% and 31.87% respectively. These results indicate that extracts of C. ambrosioides, at the concentrations used, do not present potential for the control of the R. (B.) microplus strains researched.